Fragment / Structure Based Drug Design (FBDD/SBDD)

Combining two most popular technologies in structural studies, X-ray crystallography and NMR, the Structural Biology Group has built a flexible yet efficient platform to support FBDD/SBDD services. We address different needs of our clients by choosing either X-ray crystallography or NMR. With the help of supplementary biophysics methods, such as SPR, TSA and etc, we are known for high-quality structural information and fast turnaround time.

X-ray crystallography for FBDD/SBDD:

  • Phoenix liquid handling machine for protein crystal HTS
  • Soaking-in or co-crystallization of small compounds

NMR for FBDD/SBDD:

  • 1D STD (Saturation Transfer Difference) NMR
  • 2D HSQC (Heteronuclear Single Quantum Coherence) NMR perturbation

Structural bioinformatics for FBDD/SBDD:

Antibody structural studies to support IP and/or design

Antibody structural study is an important part of drug researches. The Structural Biology group has experiences in Fab/Fab-antigen complex structure determination / modeling and fine epitope mapping of antigen-antibody interaction.

Fab/Fab-antigen complex structure

  • Papain digestion
  • Protein A column, protein C column, MonoQ column, MonoS column and SEC
  • High throughput crystal hit screening, crystal optimization and structure determination
  • Fab/Fab-antigen complex structure modeling

Fine epitope mapping

  • NMR TROSY-HSQC spectra
Lipidic Cubic Phase (LCP) crystallization services
The amazing high resolution crystal structure of β2AR in 2007 brought LCP crystallization method to the world’s attention. Ever since then, this method has led to more and more success in the area of GPCR and membrane protein structural studies. Now we are glad to announce that LCP has been added to our capability list.Using this technique, we offer our clients highest throughput screening for membrane protein crystallization and theoretically largely increased success rate. We also offer to carry out subsequent crystal optimization, crystal handling and data collection, which are known to be very difficult in LCP system.Illustration of LCP system
LCP crystal gallery
DNA to Structure
Solving novel structures for protein and protein complexes requires not only experiences but also expert thinking. With successful track records, the Structural Biology group is willing to take up challenges and solve difficult problems that our clients face.Having a dedicated protein production team within group and master knowledge of X-ray crystallography / NMR, we are able to offer services from construct design, to protein expression and purification, to structure determination and to structure analysis.
Fragment / Structure Based Drug Design (FBDD/SBDD)

Combining two most popular technologies in structural studies, X-ray crystallography and NMR, the Structural Biology Group has built a flexible yet efficient platform to support FBDD/SBDD services. We address different needs of our clients by choosing either X-ray crystallography or NMR. With the help of supplementary biophysics methods, such as SPR, TSA and etc, we are known for high-quality structural information and fast turnaround time.

X-ray crystallography for FBDD/SBDD:

  • Phoenix liquid handling machine for protein crystal HTS
  • Soaking-in or co-crystallization of small compounds

NMR for FBDD/SBDD:

  • 1D STD (Saturation Transfer Difference) NMR
  • 2D HSQC (Heteronuclear Single Quantum Coherence) NMR perturbation

Structural bioinformatics for FBDD/SBDD:

Antibody structural studies

Antibody structural study is an important part of drug researches. The Structural Biology group has experiences in Fab/Fab-antigen complex structure determination / modeling and fine epitope mapping of antigen-antibody interaction.

Fab/Fab-antigen complex structure

  • Papain digestion
  • Protein A column, protein C column, MonoQ column, MonoS column and SEC
  • High throughput crystal hit screening, crystal optimization and structure determination
  • Fab/Fab-antigen complex structure modeling

Fine epitope mapping

  • NMR TROSY-HSQC spectra
DNA to Structure
Solving novel structures for protein and protein complexes requires not only experiences but also expert thinking. With successful track records, the Structural Biology group is willing to take up challenges and solve difficult problems that our clients face.Having a dedicated protein production team within group and master knowledge of X-ray crystallography / NMR, we are able to offer services from construct design, to protein expression and purification, to structure determination and to structure analysis.
Dedicated protein production team
In order to meet special needs for various projects, we have a well-trained dedicated protein production team. With vast knowledge and experiences, we help our clients with construct optimization based on bioinformatic analysis, troubleshooting for protein expression and purification, folding and refolding, specific labeling and etc.
Currently, we are building up two exciting new capabilities:

GPCR production

  • Bioinformatic research
  • Construct design: T4 lysozyme insertion, glycosylation site mutation, N-term / C-term truncation and thermal-stable mutation
  • Protein expression in insect cell system
  • Protein purification in detergent micelle
  • QC by SDS-PAGE, Western Blotting, ASEC, DLS and etc

Isotope labeled protein production

  • Expression of isotope labeled protein (up to ~35Kda) in M9 media ( uniform labeling 15N, uniform labeling 15N, 13C and uniform labeling 15N, 13C, 2H)
  • Selective isotope-labeling method (SAIL and etc)