Metabolic & Cardiovascular Diseases

The Metabolic and Cardiovascular Team has extensive experience in management and research. We collaborate with pharmaceutical, academic and biotech companies worldwide, and our clients’ needs are our priorities. Our team provides high-quality and comprehensive services for efficacy and mechanism of action of drug candidates.

In vitro Assays

• Multiple technology and screening platforms

• Comprehensive services

• Wide range drug targets

Enzyme and inhibitor, GPCR, protein transporter, antigen and antibody, biomarker

In vivo Animal Models

Diabetes & Obesity

• Db/db mice (BKS.Cg-Dock7m +/+ Leprdb/J)
• Ob/ob mice (B6.Cg-Lepob/J)
• Zucker Diabetic Fatty (ZDF) Rats
• High-fat diet-induce obesity (DIO) mice, rats
• NOD/ShiLtJ  type 1 diabetic  mice

Measurement:

• Blood glucose, insulin
• Urine glucose, HbA1c
• Glucose tolerance test  (OGTT, IVGTT)
• Food intake and effect on body weight
• Insulin tolerance and sensitivity

Nephropathy

• Unilateral ureter obstruction (UOO) kidney fibrosis
• Uninephrectomy (Unx) Nephropathy
• Ischemia-reperfusion kidney injury
• LPS , Folic acid induced kidney injury

Measurement:

• 24 hour albuminuria, proteinuria, urine glucose
• Inflammation marker: TGF-β1, IL-6
• Glomerular Filtration Rate GFR
• Kidney function and marker: CRE, BUN, UA, Kim-1, renin
• HE or PAS pathology staining

Cardiovascular Disease

• Spontaneous Hypertensive Rat (SHR)
• Spontaneous dyslipidmia mice ApoE(-/-)；LDL(-/-)
• Arteriovenous (AV)-shunt model
• Aortic banding ,ischemia and reperfusion
• Deoxycorticosterone acetate (DOCA) + high salt induced heart  injury
• FeCl2 induced venous thrombus model
• High fat , high-cholesterol diet induced heart disease

Measurement:

• A tail-cuff system-BP2000 Blood pressure analysis
• Lipid profiling: TC, TG, LDL, HDL
• Thrombus weight
• Prothrombin time

Liver Disease

• Methionine-and choline-deficient (MCD) diet  induced fibrosis
• High fat, high cholesterol, high sucrose (HFCS) diet induced model
• Diabetic  NAFLD mouse model
• Thioacetamide TAA  induced model
• High fat diet  + carbon tetrachloride CCl4 induced fibrosis

Measurement:

• Body weight, liver weigh
• Liver histological analysis (H&E,  Sirius Red, tri chrome staining)
• liver  function -serum ALT, AST, ALP
•  Lipid profile – serum TC, HDL, LDL, TG
• Fibrosis marker- hyaluronic acid, a-SMA
• Inflammation biomarker: TNF-a, IL-1b, MCP-1, TGF-b1, IL6,IL8, sCD14
• Cell death:cytokeratin18 (CK18-30)

Case study

• Diabetes case study
  
• Cardiovascular case study
  
• Kidney disease case study