Metabolic & Cardiovascular Diseases

With well skills and experience in metabolic research field, we provide the pharmacodynamics and pharmacological study for your preclinical candidates. By using our invivo animal models of human disease, together with in vitro assay, we facilitate our customers to evaluate efficacy and mechanism of action of drug candidates. We also have strong capability to provide customized services of new assays and animal models based on the literature. Our services include but not limited to

In vitro Assays

• Anti-oxidation activity screening platforms
• Inhibitory activity assay against the PCSK9/LDLR interaction
• DPPIV inhibitory activity assay
• Glucose Stimulated Insulin Secretion assay (GSIS)

In vivo Animal Models

Diabetes & Obesity

• db/db mice
• ob/ob mice
• Zucker Diabetic Fatty (ZDF) Rats
• High-fat diet-induce obesity (DIO)
• Streptozocin/HFD+STZ induced diabetes models

Measurement:

• Blood glucose, insulin
• Urine glucose, HbA1c
• Glucose tolerance test  and insulin tolerance test (GTT, ITT)
• Food intake and effect on body weight

Nephropathy

• Unilateral ureter obstruction (UOO) kidney fibrosis model
• Diabetic nephropathy model
• Acute kidney injury (Ischemia-reperfusion)
• Ade-induced chronic kidney injury
• Chronic kidney disease with 5/6 nephrectomy in mice

Measurement:

• Glomerular Filtration Rate GFR
• 24 hour albuminuria, proteinuria, urine glucose
• Inflammation marker: TGF-β1, IL-6
• Kidney function and marker: CRE, BUN, UA, Kim-1, renin
• HE or PAS pathology staining

Cardiovascular Disease

• Hypertension: Spontaneously Hypertensive Rat (SHR) / salt induced hypertension in Dahl rats
• High fat diet induced atherosclerosis in ApoE -/- mice
• Golden hamster hyperlipidemia model

Measurement:

• A tail-cuff system-BP2000 Blood pressure analysis
• Lipid profiling: TC, TG, LDL, HDL
• En-face Oil red O staining
• Histology analysis: HE or Oil red stain

Liver Disease

• Methionine-and choline-deficient (MCD) diet induced NAFLD/NASH model
• High fat, high cholesterol, high sucrose (HFCS) diet induced NAFLD/NASH model
• High fat diet  + carbon tetrachloride CCl4 induced NAFLD/NASH model
• CCl4 induced acute liver injury and liver fibrosis
• Drug induced acute liver injury

Measurement:

• Body weight, liver weigh
• Liver histological analysis (H&E,  Sirius Red, tri chrome staining)
• liver  function -serum ALT, AST, ALP
• serum and liver lipid profile
• Fibrosis marker- hyaluronic acid, a-SMA
• Inflammation biomarker: TNF-a, IL-1b, MCP-1, TGF-b1, IL6,IL8, sCD14
• Histopathology analysis and score

Case study

• Case study: diabetes

 

• Case study: hypertension

• Case study: diabetic nephropathy

• Case study: atherosclerosis