Metabolic & Cardiovascular Diseases
In vitro Assays
- Anti-oxidation activity screening platforms
- Inhibitory activity assay against the PCSK9/LDLR interaction
- DPPIV inhibitory activity assay
- Glucose Stimulated Insulin Secretion assay (GSIS)
In vivo Animal Models
Diabetes & Obesity
- db/db mice
- ob/ob mice
- Zucker Diabetic Fatty (ZDF) Rats
- High-fat diet-induce obesity (DIO)
- Streptozocin/HFD+STZ induced diabetes models
Measurement:
- Blood glucose, insulin
- Urine glucose, HbA1c
- Glucose tolerance test and insulin tolerance test (GTT, ITT)
- Food intake and effect on body weight
Nephropathy
- Unilateral ureter obstruction (UOO) kidney fibrosis model
- Diabetic nephropathy model
- Acute kidney injury (Ischemia-reperfusion)
- Ade-induced chronic kidney injury
- Chronic kidney disease with 5/6 nephrectomy in mice
Measurement:
- Glomerular Filtration Rate GFR
- 24 hour albuminuria, proteinuria, urine glucose
- Inflammation marker: TGF-β1, IL-6
- Kidney function and marker: CRE, BUN, UA, Kim-1, renin
- HE or PAS pathology staining
Cardiovascular Disease
- Hypertension: Spontaneously Hypertensive Rat (SHR) / salt induced hypertension in Dahl rats
- High fat diet induced atherosclerosis in ApoE -/- mice
- Golden hamster hyperlipidemia model
Measurement:
- A tail-cuff system-BP2000 Blood pressure analysis
- Lipid profiling: TC, TG, LDL, HDL
- En-face Oil red O staining
- Histology analysis: HE or Oil red stain
Liver Disease
- Methionine-and choline-deficient (MCD) diet induced NAFLD/NASH model
- High fat, high cholesterol, high sucrose (HFCS) diet induced NAFLD/NASH model
- High fat diet + carbon tetrachloride CCl4 induced NAFLD/NASH model
- CCl4 induced acute liver injury and liver fibrosis
- Drug induced acute liver injury
Measurement:
- Body weight, liver weigh
- Liver histological analysis (H&E, Sirius Red, tri chrome staining)
- liver function -serum ALT, AST, ALP
- serum and liver lipid profile
- Fibrosis marker- hyaluronic acid, a-SMA
- Inflammation biomarker: TNF-a, IL-1b, MCP-1, TGF-b1, IL6,IL8, sCD14
- Histopathology analysis and score
Case study
- Case study: diabetes
- Case study: hypertension
- Case study: diabetic nephropathy
- Case study: atherosclerosis
- Service Overview
- Discovery Chemistry
- Biology & Pharmacology
- DMPK / Exploratory Toxicology
- Biologics Discovery
- Biologics CMC
- Small Molecule CMC